Continuous focusing, fractionation and extraction of anionic analytes in a microfluidic chip
نویسندگان
چکیده
منابع مشابه
Multistage isoelectric focusing in a polymeric microfluidic chip.
This paper reports a protocol that improves the resolving power of isoelectric focusing (IEF) in a polymeric microfluidic chip. This method couples several stages of IEF in series by first focusing proteins in a straight channel using broad-range ampholytes and then refocusing segments of the first channel into secondary channels that branch from the first one at T-junctions. Experiments demons...
متن کاملIsoelectric focusing in a poly(dimethylsiloxane) microfluidic chip.
This paper reports the application of ampholyte-based isoelectric focusing in poly(dimethylsiloxane) (PDMS) using methylcellulose (MC) to reduce electroosmosis and peak drift. Although the characteristics of PDMS make it possible to fabricate microfluidic chips using soft lithography, unstable electroosmotic flow (EOF) and cathodic drift are significant problems when this medium is used. This p...
متن کاملMagnetic Microparticle Based Dna Extraction in a Droplet Microfluidic Chip
We are reporting an efficient method for magnetic microparticle based DNA extraction on a segmented flow microfluidic platform [1]. The droplet splitter and the magnetic setup were optimized separately to achieve highest separation efficiency. The novel asymmetric droplet splitter was developed allowing for dynamic control and arbitrary selection of droplet splitting ratios between 50:50 and 95...
متن کاملNumerical Study of Droplet Generation Process in a Microfluidic Flow Focusing
Microfluidic flow focusing devices have been utilized for droplet generation on account of its superior control over droplet size. Droplet based microfluidics addressed many scientific issues by providing a novel technological platform for applications such as biology, pharmaceutical industry, biomedical studies and drug delivery. This study numerically investigated the droplet generation proce...
متن کاملContinuous isolation of monocytes using a magnetophoretic-based microfluidic Chip.
Monocytes play an important role in the immune system and are responsible for phagocytizing and degrading foreign microorganisms in the body. The isolation of monocytes is important in various immunological applications such as in-vitro culture of dendritic cells. We present a magnetophoretic-based microfluidic chip for rapid isolation of highly purified, untouched monocytes from human blood by...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2019
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c9lc00434c